It is clearly evident from the above findings that the test samples of A. blanchetii possess different types of bioactivities. Therefore, the plant is a good candidate for carrying out further chemical and biological studies to isolate the active principles to correlate with its biological activities. All authors MK 8776 have none to declare. “
“Metoclopramide is chemically 4-amino-5-chloro-N-[2-(diethylamino)ethyl]-2-methoxybenzamide, an antiemetic and gastroprokinetic agent. It is commonly used to treat nausea and vomiting, to facilitate gastric emptying in people with gastroparesis, and as a treatment
for gastric stasis often associated with migraine headaches. The antiemetic action of Metoclopramide is due to its antagonist activity at D2 receptors in the chemoreceptor trigger zone (CTZ) in the central nervous system (CNS)—this action prevents nausea and vomiting triggered by most stimuli. 1 At higher doses, 5-HT3 antagonist activity may also contribute to the antiemetic effect. The MK-1775 research buy gastroprokinetic activity
of Metoclopramide is mediated by muscarinic activity, D2 receptor antagonist activity and 5-HT4 receptor agonist activity. 2 Metoclopramide is freely soluble in water and ethanol and practically insoluble in ether. The molecular formula is C14H22ClN3O2, which corresponds to a molecular weight of 299.80. Very few analytical methods have been reported for the quantitative determination of Metoclopramide in formulations as well as biological fluids. These include gas chromatography3 and 4 and high performance liquid chromatography.5 and 6 These previously published methods comprise of complicated mobile systems and are not directly applicable for this novel type of dosage form which is prepared and need more investigation for method development and validation. However, no stability indicating UPLC methods were reported to estimate Metoclopramide and its degradation products (Fig. 1). The proposed method was stability indicating
by which all the degradation products of Metoclopramide PD184352 (CI-1040) can be estimated quantitatively at very low levels. Metoclopramide (purity 99.0%) and standard materials of degradation products were obtained from Hospira Health Care India Pvt Ltd, Chennai, India. Monobasic sodium phosphate, pentane-1-sulfonic acid sodium salt, orthophosphoric acid and acetonitrile were purchased from Ranbaxy Chemicals, New Delhi, India and all are of HPLC grade. Water was purified by milli-Q-water purification system (Millipore, Bedford, MA, USA) and used for preparation of all the solutions. The analysis was performed using Waters Acquity system equipped with a binary solvent delivery pump and PDA detector. Data acquisition and processing were done by using Empower2 software version FR5 (Waters Corporation, USA). The chromatographic separation was performed using a Waters X-terra RP18 column (150 × 4.6 mm), 3.5 μ particle column. The mobile phase was a mixture of mobile phase A and mobile phase B.