significant changes were measured for body mass (BM) or lean body mass (LBM) in either group. A group x time effect for total body fat percent (P=0.01; Semaxanib mouse mean ± SE; PL baseline, 42.3 ± 0.2% to post, 42.6 ± 0.2%,+ 0.71 %; MIDS baseline, 44.5 ± 0.2% to post, 43.8 ± 0.2%,-2.24%) and android fat percent (P= 0.03; PL baseline, 49.1 ± 0.2% to post, 49.3 ± 0.2%,+ 0.4%; MIDS baseline, 51.8 ± 0.3 % to post, 50.9 ± 0.3%, – 0.9%) was observed. There was a main time effect where satiety increased (P= 0.004) and desire to eat decreased (P=0.007). No other changes were reported. The side effects reported with MIDS were headache (n=1), anxiety (n=1), and jitteriness (n=1) and for PL were headache (n=1), bloated feelings (n=1), and improved bowel movements (n=1). Conclusion Eight weeks of MIDS supplementation significantly decreased total and android fat percent. A main time effect was observed for
satiety and desire to eat. Health indices of blood pressure, heart rate and blood lipids did not differ between groups. Acknowledgements This study was supported by an independent research grant from the International Society of Sports Nutrition to MJO.”
“Background An intact composition of extracellular matrix (ECM) collagens, proteoglycans and elastic fibres are responsible for the constitutional strength of tendons and ligaments [1, 2]. It is known that pathophysiological changes in the ECM could lead to reduced extension properties and diminished capacity of energy absorption of ligaments and tendons and could see more promote diseases like patellar tip syndrome, tendinopathy and rupture [3, 4]. In a clinical study it could be demonstrated that the oral ingestion of specific collagen peptides improved extension properties of the finger joints HSP90 . Aim of the present study was to investigate the impact of a specific
collagen peptide composition (FORTIGEL®) on the extracellular matrix of ligaments and Achilles tendons. check details Previous experimental studies confirmed the stimulatory impact of these bioactive collagen peptides on the ECM biosynthesis of joint cartilage tissue [6–8]. Methods Primary fibroblasts derived from human ligaments and tendons were isolated by enzymatic digestion and seeded in monolayer cultures in a humidified incubator in 5 % CO2 atmosphere at 37° C. After 80 % cell confluence regular culture medium was supplemented with 0.5 mg/ml of a specific collagen hydrolysate (FORTIGEL®, GELITA AG, Germany). The RNA expression of matrix molecules and degenerative metalloproteinases was determined via real-time PCR after a stimulation time period of 24 h. Moreover, the collagen, proteoglycan and elastin biosynthesis of tendon and ligament derived fibroblasts was determined using validated methods like western blotting, alcian blue staining or 14[C]-incorporation assay. Results The biosynthesis of ligament and tendon matrix molecules was clearly stimulated by FORTIGEL®.