The final immunoreactive score was determined by multiplying the intensity https://www.selleckchem.com/products/AZD6244.html scores with the extent of positivity scores of stained cells, with the minimum score of 0 and a maximum score of 12 [24–26]. Slides were independently examined by 2 pathologists (Chui-feng Fan and Min Song) as previously mentioned; however, if there was a discrepancy in individual scores both pathologists reevaluated together by reaching a consensus agreement before combining the individual scores. To obtained statistical results, a final score equal to or less than 1 was considered as negative, while scores of 2 or more were considered as positive.

Statistical analysis: The results were evaluated using the χ2 test. The correlation learn more between p53 nuclear accumulation and ERα expression was tested by using the Pearson chi-square test. All statistical analyses were performed using PND-1186 chemical structure SPSS 13.0 for Windows (SPSS Inc., Chicago, IL, USA). Statistical significance in this study was set at P < 0.05. All reported P values are two-sided. Results p53 nuclear

accumulation in ductal hyperplasia of breast The phenotypic expression patterns of p53 in breast ductal hyperplasia were shown in Figure 1. Table 2 showed p53 nuclear accumulation in ductal hyperplasia of breast. No p53 nuclear accumulation was found in UDH (0/79) regardless of co-existing DCIS or IDC. p53 nuclear accumulation was detectable in 22.8% of ADH (31/136), higher than that in UDH (P < 0.001), lower than that in DCIS (41.5%, 17/41) or in IDC (42.2%, 19/45) respectively (P < 0.01). No difference in nuclear p53 accumulation were observed between pure

ADH (14/77) and ADH/DCIS (9/29) (18.2% vs. 31.0%, P > 0.05) or ADH/IDC (8/30) (18.2% vs. 26.7%, P > 0.05). Figure 1 Immunohistochemical staining of noninvasive breast lesions with antibody against p53. p53 nuclear accumulation was not found in epithelial cells of normal ducts (a) and usual ductal hyperplasia (b) of breast. p53 positive staining in atypical ductal hyperplasia (c): the bigger arrow shows a breast duct filled with cells with atypical hyperplasia. The cells are quite identical in size and shape. Staining of p53 is seen in some nuclears (> 10%). mafosfamide The little arrow shows a normal duct without p53 nuclear accumulation. p53 positive staining in ductal carcinoma in situ (d): the bigger arrow shows a ductal carcinoma in situ with positive staining of p53 in nuclears (> 10%). The little arrow shows necrosis in the ductal carcinoma in situ. (× 40) Table 2 p53 nuclear accumulation and ERα expression in ductal hyperplasia of breast   Total no. p53 nuclear accumulation P-value ERα expression P-value     + –   + –   UDH                  Pure type 52 0 52 > 0.05 52 0 > 0.