Despite severe lymphopenia, no patient treated for URTID progressed to LRTID. Neutrophil recovery was delayed in 3 patients.
Conclusions. We show that IV and oral RBV were efficacious in preventing progression and reducing mortality of RSV infection in this small series of allogeneic HSCT recipients. Randomized studies are not to be expected for this condition and therefore reporting case series could help in determining optimal RSV treatment.”
“BACKGROUND: Protein stability this website of therapeutic monoclonal antibodies during processing and final storage is imperative for the commercial
success of the product. Amino acid addition is one of the options for stabilization of proteins that can be employed during the manufacturing process and storage.
RESULTS: Use of arginine in the elution buffer during Protein
A purification and subsequent neutralization doubled the yield of antibody compared with the original glycine-based elution buffer. The role of amino acids in stabilizing monoclonal antibody liquid formulations was then studied using differential scanning calorimetry. Increase in the unfolding transition temperatures (T(m)) of immunoglobulin G(IgG) was measured after supplementing a glycine buffer with a range of amino acids. The basic amino acids histidine, lysine, and arginine stabilized all three domains of IgG. The neutral amino acids serine and alanine provided less stabilization; glutamine, proline, and the acidic amino acids provided 3-MA cost negligible stabilization.
CONCLUSION: The positive charge on the side-chain of histidine, lysine, and arginine appears to be the most important factor affecting the IgG stabilization. It is probable that the mechanism for IgG stabilisation is the same for all of the basic amino acids and that it binds
transiently to IgG side chains, altering water populations in the solvation shell, making unfolding and aggregation less energetically favourable. (C) 2011 Society of Chemical Industry”
“Background: IGF-1R inhibitor Patellofemoral pain syndrome is one of the most common knee problems and may be related to abnormal patellar tracking. Our purpose was to compare, in vivo and noninvasively, the patellar tracking patterns in symptomatic patients with patellofemoral pain and those in healthy subjects during squatting. We tested the hypothesis that patients with patellofemoral pain exhibit characteristic patterns of patellar tracking that are different from those of healthy subjects.
Methods: Three-dimensional patellar kinematics were recorded in vivo with use of a custom-molded patellar clamp and an optoelectronic motion capture system in ten healthy subjects and nine subjects with patellofemoral pain. The position of osseous knee landmarks was digitized while subjects stood upright, and then patellofemoral kinematics were recorded during squatting. The tracking technique was validated with use of both in vitro and in vivo methodologies, and the average absolute error was <1.2 degrees and <1.1 mm.