Gotoh. Electronic supplementary material Additional file 1: Figure S1. Cross-streak experiment for detection of bacterial interaction via acyl-HSLs. The two monitor strains used were KG7004 (ΔlasI ΔrhlI) and KG7050 (ΔlasIΔrhlI4 ΔmexB) harboring the lasB promoter-gfp plasmid (pMQG003) were used. Test strains against the monitor strains (center) were cross-streaked on LB agar plates. Following 24 h incubation at30°C, the growth of strains was observed under a stereomicroscope, and then production of GFP by the monitor strains was visualized by excitation of the plates with blue light. (PDF 668 KB) Additional file 2: Figure S2. TLC analysis of 3-oxo-C10-HSL produced by V. anguillarum.
Extracted samples from V. anguillarum H 89 cultures were chromatographed check details on a C-18 RP-TLC plate, developed with methanol/water (70:30, v/v). The spots were visualized 13 by overlaying the TLC plate with C. violaceum VIR07. As AHL standards, Cn-HSL: 14 C6-HSL, C8-HSL and C10-HSL, 3-oxo-Cn-HSL: 3-oxo-C6-HSL, 3-oxo-C8-HSL, 15 3-oxo-C10-HSL and 3-oxo-C12-HSL were used. (PDF 389 KB) Additional file 3: Supplemental information
of Materials, Methods, Figure legend of Figure S1 and S2 and References[1, 45–49]. (PDF 329 KB) References 1. Fuqua C, Greenberg EP: Listening in on bacteria: acyl-homoserine lactone signaling. Nat Rev 2002, 3:685–695.CrossRef 2. Waters CM, Bassler BL: Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 2005, 21:319–346.PubMedCrossRef 3. Duan K, Surcttc MG: Environmental regulation of Pseudomonas aeruginosa PAO1 Las and Rhl quorum-sensing system. J Bacteriol 2007, 189:4827–4836.PubMedCrossRef 4. Schuster M, Lostroh CP, Ogi T, Greenberg EP: Identification, timing, and signal specificity of Pseudomonas
aeruginosa quorum-controlled genes: a transcriptome analysis. Histone demethylase J Bacteriol 2003, 185:2066–2079.PubMedCrossRef 5. Wagner VE, Li LL, Isabella VM, Iglewski BH: Analysis of the hierarchy of quorum-sensing regulation in Pseudomonas aeruginosa. Anal Bioanal Chem 2007, 387:469–479.PubMedCrossRef 6. Bottomley MJ, Muraglia E, Bazzo R, Carfi A: Molecular insights into quorum sensing in the human pathogen Pseudomonas aeruginosa from the structure of the virulence regulator LasR bound to its autoinducer. J Biol Chem 2007, 282:13592–13600.PubMedCrossRef 7. Dubem JF, Diggle SP: Quorum sensing by 2-alkyl-4-quinolones in Pseudomonas aeruginosa and other bacterial species. Mol Biosyst 2008, 4:882–888.CrossRef 8. Pearson JP, Gray KM, Passador L, Tucker KD, Eberhard A, Iglewski BH, Greenberg EP: Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci USA 1994, 91:197–201.PubMedCrossRef 9. Bredenbruch F, Geffers R, Nimlz M, Buer J, Haussler S: The Pseudomonas aeruginosa quinolone signal (PQS) has an iron-chelating activity. Environ Microbiol 2006, 8:1318–1329.PubMedCrossRef 10.