The method has been applied selleck compound to the embryos or axes of three oilseed species (from the Mediterranean to the tropics) with lipid melting properties that span −45 °C to 35 °C (Nadarajan and Pritchard,

2014). As eluded to above, further integration of ex situ and in situ conservation strategies is an increasing necessity ( Volis and Blecher, 2010), as is consideration of circa situ conservation approaches, e.g. in smallholders’ agroforests ( Dawson et al., 2013). The trees planted in such agroforests can act as reservoirs of biodiversity, provide alternative sources of product to wild harvesting and may stimulate species inclusion in seed collections or field gene banks. Tropical forests selleck chemical are typically diverse and comprise few abundant species and a large number of rare species which may be represented by less than one individual per hectare, as a consequence

of differential survival of seedlings based on density-dependent (e.g., competition, vulnerability to herbivores) and other effects (Chave et al., 2006). Consequently, seed supply for ex situ programmes may be limited. Moreover, many of the species of interest produce difficult to handle, highly recalcitrant seeds. Developing the horticultural skills to handle seedlings can, therefore, provide additional opportunities to support conservation. One example is the use of seeds to produce seedlings to be planted out as framework species

for the restoration of forests. The primary purpose of this group of species C1GALT1 is rapid growth and accelerated regeneration at that site through the dispersal process, thus enhancing habitat heterogeneity ( Tucker and Murphy, 1997). Out of 37 species native to northern Thailand trialled, nine were ranked as excellent framework species to accelerate the natural regeneration of the forest ecosystem and encourage biodiversity recovery on degraded sites ( Elliot et al., 2003). Another example that demonstrates the scale of the horticultural task is that regulations governing re-afforestation programmes in degraded areas in South Brazil prescribe the use of a minimum of 80 species, distributed across pioneers and non-pioneers, and with these accounting for at least 40% of the total number of species; also any of the planted species cannot exceed 20% of this total (Camargo, J.L.C., pers comm.). Restoration efforts involving native tree species are discussed in detail elsewhere in this special issue ( Thomas et al., 2014). In situ conservation of seedling banks could be a means of maintaining a large number of young plants in a convenient reduced space. However, longer-term maintenance would be dependent upon restricting light levels to just above the compensation point, or limiting nutrition, to reduce plant growth, without stressing the plants.