Protein in skeletal muscle fibers AUY922 747412-49-3 exposed stretch.15 obtained Ht, 16 HIF-1 is overexpressed in VSM cells from rat heart and the aorta to the mechanical stretch.17, exposed to 18 when the regulation of oxygen dependent Ngig HIF occurs level of protein stabilization, the induction of HIF occurs by mechanical strain in the transcription of the gene and translation, and probably involves the phosphatidylinositol 3-kinase and protein mitogenactivated kinase.8, 15 Search mechanisms upstream in the regulation of contraction and ofMMPexpression in veins reduced under constant strain are involved, to this study the hypothesis that the increase to test the vein wall associated with the voltage overexpression of HIF 1 and HIF 2, resulting in the Erh increase the expression of specific MMP and a decrease in the curves sen contraction. We have to see the rats IVC whether L Ngere Erh Relationships in the blood vessel wall with an increased Associated Hten expression of HIF 1 and HIF 2 when the obtained Hte expression of HIF in the veins is associated with Stretched increasedMMPexpression and a decrease in the curves sen contraction and, if the expression of HIF blocked or prevented that the machine took overexpression of MMPs and improves the tensile strength of contraction in the veins. METHODS L Solutions and drugs. Cancer-L Solution contained NaCl 120, KCl 5.9, NaHCO3 25, NaH2PO4 1.2, dextrose 11.5, CaCl 2 2.5, MgCl 2 and 1.2, with 95% O2 and 5% CO2 bubbled at pH 7 , 4th Membrane depolarization by KCl stimulated VSM contraction high extracellular Ca2 receipt of Ren matrix space.19 high KCl-L Was produced as a depolarizing solution induced cancer Equimolar substitution of NaCl with KCl. Adrenergic phenylephrine also used to stimulate the contraction IVC. Tissue culture medium was used to incubate the veins in the night and was minimum essential medium with penicillin, streptomycin and amphotericin B erg Composed complements drugs that were inhibiting the expression / activity T of HIF U0126, 17 DMAG ethyl] amino desmethoxygeldanamycin 17 and echinomycin. Dimethyloxallyl glycine was used to inhibit protein degradation and hydroxylation of HIF FIH byPHDand. Animals and tissues. M Nnliche Sprague Dawley rats were get by CO2 inhalation Tet.
The Bauchh cave was GE Opened, and the inferior vena cava was cut, placed in Cancer, cleaned of tissue under the microscope of the weed adissecting, and four servings, 3 mm wide rings. All procedures followed the guidelines of the Animal Care Committee at Harvard and use. Isometric contraction. Each segment of IVC was placed between two metal hooks in a water bath fabrics tungsten double envelope filled with 50 ml of Krebs aufgeh Depends bubbled with 95% O2 and 5% CO2 at 37th A hook was attached to a glass rod at the bottom of the bath tissue, and the other hook is connected to a Grass force transducer, and amendments were Ver In isometric contractions recorded on Grass polygraph. Partial pressure of oxygen in the tissue bath was kept constant, so that Changes in HIF-1 and HIF-2 expression to Ver Changes the voltage connected to the vein wall is in all experiments. Since HIF is known to be regulated by oxygen tension, should future experiments to determine whether the Change of tissue oxygen partial pressure in the bath are also on the contraction of the visible veins prolonged stretch. We have previously shown that rats instance, increases basal ten ht IVC underwent.