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“The reductive biotransformation of alpha-, beta-, gamma, and delta-hexachlorocyclohexane isomers was investigated using five alternative electron donors (i.e., glucose plus rnethanol, glucose only, methanol only, acetate, and ethanol) in

a batch assay of an HCH-dechlorinating anaerobic culture. In addition, Selleckchem RSL-3 a life cycle assessment was conducted using the IMPACT2002+ method to evaluate the environmental effects of HCH bioremediation with the aforementioned electron donors. Results showed that the electron donors methanol plus glucose, ethanol, glucose, and methanol can significantly enhance the biotransformation of each HCH isomer. However, the amended electron donors and the byproduct of the MEK162 anoxic/anaerobic systems may negatively affect the environment (e.g., respiratory inorganic, land occupation, global warming, and non-renewable energy categories). These effects are attributed to the electron donor production processes. To avoid secondary pollutants, a linear relationship between the upper bound electron donor applications and HCH concentration was observed from an environmental perspective. Results indicated

that the methanol scenario was the most suitable option for the current research. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.”
“Background/Aims: Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is a rare renal tubular disorder complicated by progressive renal failure during childhood or adolescence. Recently, causative mutations in the CLDN19 gene have been identified in FHHNC patients presenting with severe ocular involvement. The aim of the study was to investigate the molecular genetic defect VEGFR inhibitor underlying FHHNC in a consanguineous Pakistani family. Methods: Clinical and biochemical parameters of the proband were studied during the follow-up period over 5 years. Genotyping of 7 members of the family was performed by amplifying microsatellite markers, tightly linked to the CLDN16 and CLDN19 genes. The two genes were sequenced directly in an automated sequencer. PCR-RFLP assay and bioinformatic

analysis were performed to verify the identified mutation. Results: Genotyping revealed that the proband was homozygous for the marker loci tightly linked to the CLDN19 gene. Sequence analysis in the proband revealed homozygosity for a novel missense mutation in exon 3 of the CLDN19 gene (389G > A) resulting in G130D amino acid substitution. Bioinformatic analysis supported the pathogenicity of the identified mutation. Family screening revealed nephrolithiasis in 3 of 6 (50%) heterozygous carriers of the pathogenic mutation. Conclusion: This study supports the fundamental role of claudin 19 for magnesium homeostasis, normal tubular structures in the kidney, and undisturbed organization and development of the retina. Copyright (C) 2011 S.