The ACE gene has

an insertion/deletion (I/D) polymorphism, which is due to the presence or PKC412 cell line absence of a 287 base pairs (bp) fragment inside intron 16. The D allele is associated with higher circulating and tissue ACE levels and low response to ACE-I and ARB medications [89,90]. These findings, however, appeared inconsistent, and the studies have been criticized because the effect on some outcomes has been modest in larger studies, suggesting a significant publication bias [91]. In addition, recent evidence suggests that the DD genotype is associated with a lower erythropoietin requirement in continuous ambulatory dialysis patients [92]. Thus, because the ACE I/D polymorphism may be a reliable and cost-effective tool to identify Lapatinib in vivo patients at risk and those who may benefit

from these therapies, and to design clinical trials in progressive nephropathies, the necessity to design additional research projects to evaluate these important issues more effectively seems unquestionable [93,94]. Although pharmacogenetic approaches, involving a single gene or a specific pathway, had reasonable success in identifying genetic variants linked to specific pharmacological phenotypes (e.g. drug metabolism, the mechanisms of action of drugs, adverse drug effects), they do not represent the gold standard, being the overall pharmacological effects of medications, and not typically monogenic traits [12]. Thus, instead of searching for a ‘dramatic genetic effect’ produced by one gene, it is more realistic to consider a group Selleck Docetaxel of genetic variants, each with a moderate effect, which together result in an

overall genetic effect in drug efficacy or toxicity. Such polygenic traits are more difficult to elucidate in clinical studies, especially when a medication’s metabolic fate and mechanisms of action are defined poorly. The completion of the Human Genome Project [95,96] and the development of innovative high-throughput screening technologies [including massive parallel gene analysis, DNA sequencing and synthesis and single nucleotide polymorphism (SNP) genotyping] have provided powerful tools to evaluate the multi-genetic influence to a specific drug therapy [21–23]. Several commercial techniques are currently available and researchers may choose the most appropriate platform to use in their projects. Among them, the DNA microarray (also referred to as gene or genome chip, DNA chip or biochip) represents the most utilized technique. This consists of an arrayed series of thousands of microscopic spots containing DNA oligonucleotide probes. The probes usually represent a short sequence of a gene specifically hybridizing a cDNA or cRNA sample (target) under high-stringency conditions.