Streptococcus pneumoniae produced three bands at 55, 150 and 200 bp (Fig. 5a, lane 3). Streptococcus agalactiae (lane 2) and S. suis (lane 4) gave similar pattern. Thus, the LAMP products of S. agalactiae and S. suis were further digested with HaeIII. The result showed that S. agalactiae was digested into four bands at 70, 216, 254 và 292 bp (Fig. 5b, lane 6), while S. suis was not digested by HaeIII (Fig. 5b, lane 5). To our knowledge, this is the first study that developed a broad range LAMP assay for simultaneous detection of more than four different bacterial species. The sensitivity of our LAMP assay was 100–1000 times higher compared with the conventional PCR assay. The
bacterial species could be distinguished among S. pneumoniae, S. suis, S. agalactiae and S. aureus based on
the digested pattern Pifithrin-�� of the LAMP products with restriction enzymes of DdeI and HaeIII. In addition, our method has learn more several advantages over the current diagnostic methods. Firstly, the method is rapid (c. 1 h) as compared with the real-time PCR method which requires 6 h to run (Nadkarni et al., 2002). Secondly, the LAMP method does not require expensive fluorimeter and fluorogenic primers and probes. Thirdly, the assay is simple and does not require highly experienced technician. More importantly, the assay can be performed in a water bath at bedside or in rural areas. These advantages suggested that our broad range LAMP assay would improve the early diagnosis and treatment of BM, helping to reduce morbidity and mortality.
Furthermore, the assay could detect bacterial species, helping to select an appropriate antibiotic therapy. One limitation of our LAMP assay was that only four species could be detected. A single-tube LAMP assay for the detection of more than four species is under development using a mixture current broad range LAMP primers and specific LAMP primers of other bacteria species. Additional Non-specific serine/threonine protein kinase clinical studies are also required to validate this new assay. Four common pathogen of BM including S. pneumoniae, S. suis, S. agalactiae and S. aureus could be simultaneously detected using a broad range LAMP assay in single tube in < 1 h. The assay is highly sensitive, rapid and simple and can be performed at bedside in healthcare facilities. We thank Dr Toru Kubo, from Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan, for his technical advice. The authors declare no competing interests of the manuscript due to commercial or other affiliations. This study was supported in part by Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) for K.H. N.T.H and L.T.T.H. contributed equally to this work. "
“The extracellular haem-binding protein from Streptomyces reticuli (HbpS) has been shown to be involved in redox sensing and to bind haem. However, the residues involved in haem coordination are unknown.