SID values are given for the combined European dataset (all isolates), for the Scottish isolates and for the isolates from mainland Europe. When comparing SIDs, differences were considered statistically significant when there were no overlaps between the confidence intervals. The phylogenetic relationships between the isolates are shown in Figure 1 using PFGE data. Distribution among different host species Map isolates from three domestic species of ruminants and 14 different wildlife species, a feral cat and a captive giraffe were typed (Table 1 and see supplementary dataset in Additional file 1

and Additional file 2: Table S3). The wildlife encompassed both ruminant and non-ruminant species. Among the wildlife

PF-02341066 mouse species, feral cat and captive giraffe, a total of nine IS900-RFLP, nine PFGE and six INMV types were detected. Dabrafenib In order to make a preliminary assessment of transmission dynamics, the combined typing data from all three molecular techniques was considered, as this was most discriminatory. A total of seven combined profiles were detected in isolates from more than one host species ([1-1], INMV1, C1; [1-1], INMV2, C1; [2-1], INMV1, C1; [2-1], INMV1, C17; [2-1], INMV2, C1; [2-19], INMV2, C5; [3-2], INMV1, C17) (Table 1). The evidence for interspecies transmission is more compelling if the same strain types are isolated from multiple species on the same property. Even with the limited data available on the properties from which the isolates in the study were obtained, it was possible to show that two combined profiles ([2-1], INMV1, C17 and ([2-19], INMV2, C5) were found in more than one species on the same property in seven cases (Table 5). Of these, four properties included isolates from both livestock

and wildlife (EN, DR, I and R). The properties CF, DR and I, are all located within the geographical area of Perth and Kinross and EN, GE and R in the adjacent region of Angus in Scotland. Isolates from species why on all six of these properties had the same combined profile ([2-1], INMV1, C17). Profile [2-19], INMV2, C5 was obtained from a goat and a sheep on the same property in Greece. Table 5 Map strain types infecting multiple host species on a single property Property Typing profile Species EN [2-1] INMV1 C17 Cow, hare, rabbit, rook, stoat CF [2-1] INMV1 C17 Crow, fox, rabbit (5) DR [2-1] INMV1 C17 Cow, rabbit (4), woodmouse GE [2-1] INMV1 C17 Fox, stoat (2), weasel I [2-1] INMV1 C17 Rabbit, sheep R [2-1] INMV1 C17 Cow, rabbit KV [2-19] INMV2 C5 Goat, sheep Numbers in parenthesis indicate the number of animals of that species identified with the given typing profile Limited data was available for two properties in the Czech Republic, KRH and VO.