Ents were HUVEC monolayers with 10 5 M Candesartan pretreated. After 48 h of concurrent treatment, reduces the number candesartan signiantly determined. Forty-eight hours of ANG II (1 ) or VEGF (1 ng  ml) treatment induced an increase in PV-1 mRNA Signiant (Fig. 7A). Some took Ma Signaling in ANG II-dependent Ngigen PV-1 mRNA  Proteasome Inhibitors expression were involved, examined with reference to FIG. 4th Atomic force microscopy (AFM) of contr the were grown on Millicell chambers treated for 48 hours the same concentration of ANG signiantly in Endothelpermeabilit t hen 40-kDa dextran-FITC (as measured by increased contr. The r The AT1 receptor in Ang II-induced increased Hte Durchl Permeability was investigated. HUVEC monolayers were pretreated with 10 5 M Candesartan treatment for 3 inutes before to 48 h to 10 7 M ANG II.

Signiantly candesartan blunted the Durchl permeability response in the folded receptors change the permeability t by VEGF-induced HUVEC monolayers were incubated with 50 nM ZM-323881 induced for 3 in, 48 h inhibited pretreated prior to treatment with 1 ng  ml VEGF-ZM 323881, the reaction signiantly permeability t by VEGF (VEGF. 16.4 10 6 cm  s 0.7 10 6, ZM-VEGF 323 881 12.9 10 impedance measurements 6 cm  s 1.2 10 6, P 5) (Figure 1B, right) The electrical impedance HUVEC monolayers with the ECIS was trilostane measured and as an indicator of permeability ts change another 2 h to reach a stable state before treatment with ANG II (10 7 M) or VEGF (1 ng  ml) began , was it born a continuous decrease in the electrical impedance at the difference in the United controlled changes was on. impedance performance in Ang II and VEGF-treated groups on the baseline at 48 h decreased compared signiantly (ANG II 0.83 II and candesartan, as in the AFM experiments, VEGF therapies for AFM studies, the effect 25 treated ng.  ml (6.5 10 1 ), and 1 ng  ml (2.6 10 9 M). At the end of treatment were prepared the cells for AFM studies. If so, VEGFR-2 receptor antagonist ZM-323881 (50 nM) (synthesized at the Budapest University of t for technology and economy, faculty t of Chemistry and Bioengineering, Hungary) and p38 MAP kinase.

SB-203580 (1 ) were added to the cells 3 inutes prior to treatment with VEGF. for permeability tsmessungen HUVEC grown on Millicell chambers for 48 h with the same concentration of VEGF and treated ZM-323 881 in the AFM experiments. Ethics All experimental protocols were approved by the Ethics Committee of Semmelweis University t, Budapest. Visualization for statistical analysis and morphology of HUVEC with TEM after ANG II treatment m possible morphological Ver changes in HUVEC studied monolayer as a result of ANG II treatment. Transmission All data presented are the means SE. were the smooth muscle data by one-way ANOVA, followed by LSD-test analyzes. Signiance of P 5 was adopted. RESULTS ANG II and VEGF permeability t of HUVEC monolayers to investigate the effects of ANG II on HUVEC monolayer permeability t, the cells were incubated with 10 -5 M, 10 6 M, 10 7 M, Fig discussed second Effects of ANG II or VEGF treatment on the electrical impedance. HUVEC monolayers were treated with vehicle or July 1 ANG II .