stroke dmge Simir tther members in the MMP cogense cn brekdown cogengetin structuresin previous in vitro studies hs been shown to ceve proMMP to ctive MMP, which cn sccur foowing MMP.With regrd to this study, we fou tht PDGFR suppression significnty reduced MMP ctivity but not MMP. We sbserved tht the expression of MMPMMP were Osthole significnty decresed foowing PDGFR suppres sion.of these resuted in preservtion of the BBB integ rity. In , tken together, MMPs my be the direct down strem proteins of the PDGFR p pthwydirect meditors of BBB impirment foowing ICH. We hve discussed downstrem orchestrtors of PDGFR iuced BBB dmge.

We so wnted to investigte wht ws responsibe for the upstrem regu tion of PDGFR signing foowing ICH injury. Previ ous iterture hs uded to the notion tht ombin regutes the Lenalidomide expression of PDGF ough PR receptor fou in eothei ces.Therefore, in the present study, different mice modes were coucted to investigte the potenti retionship between ombinPDGF. First. in the utoogous rteri bood iuced ICH mode, we fou tht PDGF expres sion ws significnty downreguted foowing the deiv ery of hirudin, ombinspecific inhibitor. We so fou tht the effects of hirudin on BBB preservtion were reversed by exogenous PDGF injection. In the ombin injection mode, we first fou the increse of phosphoryted PDGFR eve s we s its downstrem signs, p MPKMMPs,the diminishmentGeneGenebnk ccession no. P J BSP NM_ Cbf F PPR g NM_ GDPH B Primer sequence forwrdreverse. Product size bpbe . PCR primer sequencescyce coitions. Note : P, kine phosphtse; BSP, bone sioprotein; Cbf, corebiing fctor ;

PPR , peroxisome proifertor ctivted receptor gmm ; GDPH, gycerdehydephosphte dehydrogense. Pnx notoginseng sponins The contents of the five min ingredients of Pnx notoginseng sponins re notoginsenoside R  ginsenoside Rg , ginsenoside Re  ginsenoside Rbginsenoside Rd  respectivey . Chemic purity boutws provided from WuZhou MK-8669 AP23573  Phrmceutic Group, Wuzhou, Chin tor μgm. Ce vibiity ws ssessed by the tetrzoiumbsed semiutomted coorimetric, dimethythizoy,diphenytetrzoium bromide MTT reduction ssy Sngon Biotech, Shnghi, Chinbsorbnce ws red t nm using microtiter pte reder KHB bsystems Wescn K, Fin. For iuction of osteogenic differentition, bone mrrow strom ces were seeded t density of ×cescm in cm cuture disheswere grown uer osteogenic iuction mediumOIM in Dubecco’s modified ege medium DMEM Gibco, Githerurg, MD suppemented wit μM dexmethsone, μM scorbte cid,mM gycerophosphte sodium. In ddition, ces were treted with Pnx notoginseng sponins tor μgm. Furthermore, bone mrrow strom ces uer osteogenic iuction were treted with μgm Pnx notoginseng sponins in the presence or bsence of μM PD, μM , or μM SP.

For determintion of kine phosphtse ctivity, bone mrrow strom ces uer osteogenic iuction were treted with , or μ gm Pnx notoginseng sponinskine phosphtse ctivity today , post MK-8669 572924-54-0 tretment ws determined s previousy describedby mesuring pnitropheny phosphtews normized ginst tot ceur protein contentexpressed s nmominmg protein. dditiony, mineriztion of bone mrrow strom ces ws determined by izrin red S stining t dy post tretment with , or μ gm Pnx notoginseng sponins s previousy described .Ccution of izrin red S concentrtions were modified s previousy describedby prison with n izrin red S dye strd curveexpressed s nmom fter normiztion ginst the tot ceur proteinexpressed s nmoμg protein. The bove experiments were performed t est ee times iepeentyech physicians experiment ws crried out in qudrupicte. Reverse trnscriptionpoymerse chin rection RT PCR Tot ceur RN ws isoted from bone mrrow strom ces using Tot RN Kit foowing the mnufcturer ’s remeed protoco Tingen .