In contrast to the IgE production, a significant positive dose-response relationship was found for IgG1 in 6-week-old mice. The same was observed in the 20-week-old mice, although the 10-μg dose Vismodegib nmr was not significantly higher than

the 0.1-μg dose. An effect of age on IgG1 production was seen only for the 0.1-μg dose. Six- and 20-week-old mice responded with significantly higher IgG1 levels compared with 1-week-old mice (* in Fig. 1C). No difference in IgG1 production was observed between the oldest age groups. Significant dose and age interactions were found for IL-4, -5, -10, -13 and IFNγ (Table 2). The results of the post hoc tests are shown in Fig. 2A–E. Also, significant dose and sex interactions were found for IL-5 and IL-13. The results of the post hoc tests are shown in Fig. 2F, G. The effect of the dose and age interaction was comparable for all TH2 cytokines (Fig. 2A, B, D, E). In 1-week-old mice, a significant TH2 cytokine secretion was only induced in mice immunized with the 10- μg dose. A positive dose–response relationship was also found for TH2 cytokine secretion in 6-week-old mice. However, in 20-week-old MAPK Inhibitor Library high throughput mice, the 10-μg dose tended to

give lower responses than the 0.1-μg dose. Remarkably, IFNγ (Fig. 2C) was only produced at significant levels in 1-week-old mice immunized with 10 μg OVA. An effect of age was observed also for cytokine release. For the 0.1-μg dose groups, the TH2 cytokine levels increased with age (*

in Fig. 2A, B, D, E). This was opposite for the 10-μg dose groups, where both TH2 cytokine and IFNγ secretion decreased with age (# in Fig. 2A–E, except for IL-5, where P = 0.08). As mentioned, a significant sex and dose interaction was found for IL-5 and IL-13 (Fig. 2F, G). For both males and females, there was a significant positive dose–response relationship between IL-5/IL-13 secretion and immunization dose. Female and male mice differed significantly only after immunization with the 0.1-μg dose, where females had significantly higher IL-5 secretion (‘S’ in Fig. 2F) and tended to have higher IL-13 (P = 0.08) secretion than males. The sex of the mice did not influence any Progesterone of the cell types investigated in BALF. Immunization dose or age did not affect the total number of macrophages and neutrophils (data not shown). There was a significant effect of age on the number of epithelial cells (P = 0.035), but the post hoc test only revealed a near-significant lower number in 1- compared with 6-week-old mice (P = 0.06, data not shown). A significant dose and age interaction was found for both lymphocyte and eosinophil numbers (Table 2). In 1-week-old mice, a significant cell influx in BALF was only found following immunization with the 10-μg dose (Fig. 3A, B). Comparably to the IgE production, the 0.1 compared with the 10-μg dose induced higher lymphocyte and eosinophil numbers in the 6- and 20-week-old mice (Fig. 3A, B).