Forty Chk inhibitor (50%) of the 80 serotypes encompassing Erastin solubility dmso atypical EPEC were associated with strains carrying one or more of the EHEC-plasmid genes ehxA, katP, etpD, espP. EHEC-plasmid genes etpD (p < 0.01), ehxA (p < 0.001) and espP (p < 0.001) were significantly more frequent among strains (89/129 = 69%) and serotypes (28/40 = 70%) belonging to Cluster 1 than in strains (32/106 = 30.2%) and serotypes (15/46

= 32.6%) of Cluster 2 (data not shown). Presence of virulence genes in STEC and apathogenic E. coli strains The 52 STEC strains investigated in this study belonged to 20 different serotypes (Table 2). Twelve of these (O113:H4, O113:H21, O118:H12, O146:H28, O153:H25, O174:H8, O22:H8, O22:H16, O76:H19, O8:H19, O91:H10 and O91:H21) were previously described from isolates of human origin [3]. Apart from

stx-genes, 33 (63.5%) of 52 STEC were positive for one or more of EHEC-plasmid associated genes ehxA, espP and katP. None of the STEC was positive for the plasmid etpD gene as for all other nle-genes investigated in this study (Table 1). The 21 apathogenic E. coli strains belonged to 18 different serotypes (Table 2) and were negative TPCA-1 for all virulence markers investigated in this study (Table 1). Discussion The concept of molecular risk assessment [24] has been successfully employed for grouping STEC strains into those that are associated with outbreaks and life-threatening disease in humans and those which cause less severe or are not implicated in human disease. The presence of non-LEE effector

Interleukin-3 receptor genes encoded by O-islands OI-122, OI-71 and OI-57 has been shown to be highly associated with EHEC strains that were frequently involved in outbreaks and severe disease in humans [4, 16, 17, 24, 28, 29]. In a previous work, we were able to associate the presence of OI-122 and OI-71 encoded genes with an “”EHEC-Cluster”" comprising forty-four EHEC strains as well as eight of twenty-one EPEC strains investigated [17]. This finding indicates that some EPEC strains are more related to EHEC in their virulence patterns, than others. In order to explore this relationship between EPEC and EHEC more closely, we investigated larger numbers of strains and serotypes of typical and atypical EPEC for thirteen virulence genes associated with EHEC O157 O-islands OI-122, OI-71, OI-57, the EHEC-plasmid and prophage CP-933N. Genes for nleG5-2 and nleG6-2 were included since OI-57 specific genes were previously found to be associated with classical EHEC and also with some EPEC strains [24, 28].