In comparison, PTEN staining of adjacent non-cancerous tissues was stronger and more common than that of SSCCs (IHC, 400X). B. Fluorescent-IHC clearly demonstrates that strong expression of DJ-1 is found in cytoplasm of SSCC tumor cells, while poor staining of PTEN was observed in cytoplasm of SSCC tumor cells, and that strong expression of PTEN is found in cytoplasm of adjacent non-cancerous cells, while poor staining of DJ-1 was observed in cytoplasm of adjacent non-cancerous cells

(IHC, 400X). C. Kaplan-Meier curves with univariate analyses (log-rank) comparing tumors with low- grade DJ-1 expression with those with high-grade DJ-1 expression. Patients with selleck compound low-grade DJ1 expression had a cumulative 5-year survival rate Epigenetics inhibitor of 88.0% compared with 53.9% for patients selleck chemicals llc with high-grade DJ-1 expression. Table 2 DJ-1 and PTEN expression in adjacent non-cancerous tissues and SSCCs   DJ-1 expression,n (%) PTEN expression,n (%) Total Absent Low High Absent Low High SSCC 6 (11.5%) 12 (23.1%) 34 (65.4%) 28 (53.8%) 16 (30.8%) 8 (15.4%) 52 Normal 22 (52.4) 11 (26.2%) 9 (21.4%) 4 (9.5%) 10 (23.8%) 28 (66.7%) 42 DJ-1: χ2 = 22.917; df = 2; P = 0.000. SSCC, supraglottic squamous cell carcinoma. PTEN: χ2 = 29.769;

df = 2; P = 0.000. Table 3 Relationship between DJ-1 expression and various clinicopathological factors Characteristic All cases DJ-1 Low-grade DJ-1 High-grade P All carcinomas 52 18 MycoClean Mycoplasma Removal Kit 34   Age       1.000  ≤ 61 25 9 16    > 61 27 9 18   pT status       0.003  Tis-2 15 10 5    T3-4 37 8 29   pN status       0.009  N0 24 13 11    N1-3 28 5 23   UICC stage       0.022  0-II 10 7 3    III-IV 42 11 31   Histological grade       0.758  G1 17 5 12    G2-3 35 13 22   DJ-1 is a prognostic marker for SSCC In univariate survival analysis, cumulative survival curves were calculated according to the Kaplan-Meier method (Table 4). Differences in survival

were assessed with the long-rank test. The conventional prognostic parameters pT status, lymph node status, and disease stage according to UICC reached significance for overall survival. DJ-1 positivity was associated with overall survival (P = 0.007). Figure 1C illustrates the impact of DJ-1 expression on survival times. Table 4 Univariate survival analyses (Kaplan-Meier): survival time of all patients with SSCC according to clinicopathological factors and DJ-1 expresion Overall survial Characteristic No.of cases No.of events 5-year survival Rate ( ± SE) P DJ-1 expression       0.007  Low-grade 18 7 88.0 ± 8.0    High-grade 34 21 53.9 ± 5.7   Age       0.244  ≤61 25 11 72.2 ± 7.9    > 61 27 17 58.5 ± 7.0   pT status       0.037  Tis-2 15 5 87.0 ± 10.3    T3-4 37 23 57.5 ± 5.5   pN status       0.042  N0 24 12 76.0 ± 7.7    N1-3 28 16 52.8 ± 5.6   UICC stage       0.027  0-II 10 3 99.5 ± 8.4    III-IV 42 25 58.5 ± 5.4   Histological grade       0.597  G1 17 9 68.9 ± 9.4    G2-3 35 19 62.8 ± 6.