\n\nDesign: Analysis of preclinical and clinical studies.\n\nMethods: Inanimate and cadaver dissection studies and clinical

implementation were pursued. A 3-arm RFT technique with a 30-degree offset base location proved optimal. Supine positioning with arms tucked and the patient in slight Trendelenburg position facilitated the dissection of the optical pocket. Demographic and surgical data that have been obtained and considered include patient age, sex, body mass index, pathology, and complications.\n\nResults: A series of consecutive RFT procedures has been accomplished in a limited population of patients. All cases were completed robotically with no conversions to open surgery necessary. All but the first case was accomplished on a drainless, outpatient basis.\n\nConclusions: A RFT technique that is gasless and uses a single access port in the postauricular CBL0137 solubility dmso crease and occipital hairline location is feasible, technically less challenging than other remote access buy Buparlisib methods, and safe.

Further study in an expanded patient population and in additional high-volume thyroid centers is warranted. See the videos, Supplemental Digital Content 1, http://links.lww.com/SLE/A36 and Supplemental Digital Content 2, http://links.lww.com/SLE/A37.”
“Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic ‘barcode’ that can be recovered by DNA sequencing. Our method is

a two-component system comprised of a genetic barcode cassette whose fragments are shuffled by Rci, a site-specific DNA invertase. The system is highly scalable, with the potential to generate theoretical diversities in the billions. We demonstrate the feasibility of this technique in Escherichia coli. Currently, this method could be employed to track the dynamics of populations of microbes through various bottlenecks. Advances of this method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.”
“IMPORTANCE In recent decades, there has been an increase in the number of women practicing medicine. We believe this shift may be reaching academic selleck publications in ophthalmology and changing gender trends. OBJECTIVE To determine whether there has been an increase in women publishing academic articles and editorials in ophthalmology during the past decade. DESIGN, SETTING, AND PARTICIPANTS In this observational retrospective study, 3 ophthalmology journals were sampled from 2000 and 2010 for at least 100 articles per journal, per year, and all editorials published in both years. Data reviewed included the authors’ gender, number of authors, number of references, subspecialty, and country of origin. EXPOSURE Publication by women authors.