Expression of Ku70 and Rad51 proteins increased (12 hours) after X-ray irradiation compared with no treatment control. 17-AAG itself did not change these protein expressions, but decreased the above-stated X-ray induced increase of Ku70 and Rad51 protein when combined with X-ray irradiation. No apparent change of Ku70 and Rad51 protein levels was detected between carbon beam irradiation alone and the combination with 17-AAG. Conclusions: 17-AAG showed radio-sensitization in cell killing of the oral squamous cell line when combined with X-ray irradiation. Carbon beam irradiation Dabigatran treatment is more effective than chemoradiotherapy for head and neck cancer treatment. Author Disclosure: A. Musha: None. Y. Yoshida: None. T. Takahashi: None. K. Ando: None. T. Funayama: None. Y. Kobayashi: None. A. Negishi: None. S.
Yokoo: None. T. Nakano: None. 3059 Late Rectal Injury and Microangiopathy after Irradiation: A Time Sequential Study in Rats H. Doi 1 , N. Kamikonya 1 , Y. Takada 1 , M. Fujiwara 1 , H. Miura 1 , H. Inoue 1 , M. Tanooka 1 , T. Shikata 2 , T. Tsujimura 3 , S. Hirota 1 1 Department of Radiology, Hyogo College of Medicine, Nishinomiya City, Japan, 2 Department of Pharmacy, The Hospital of Hyogo College of Medicine, Nishinomiya City, Japan, 3 Department of Pathology, Hyogo College of Medicine, Hyogo, Nishinomiya City, Japan Purpose/Objective(s): The purpose was to establish an experimental model of late radiation proctitis, and to examine an assess- ment strategy of late radiation proctitis. Materials/Methods: A total of 57 Wistar rats were used. Forty-five of the rats were exposed Dabigatran 211914-51-1 to selective rectal irradiation with a single fraction of 25 Gy. These rats were sacrificed at the 4th, 12th, 24th, and 37th week following irradiation.
The remaining 12 rats comprised the control group without irradiation. The rectal mucosal changes of each rat were evaluated macroscopically and pathologically. In the pathological examination, the mucosal changes were evaluated regarding the epithelial changes, squamous metaplasia, heteromorphic structures, and the vessels. The absolute number of vessels in the rectal mucosa was counted micro- scopically. In addition, the vascular stenosis was evaluated. 1
A62 Program and Abstracts / Antiviral Research 74 (2007) A197 84 nous retrovirus (PERV) from donor organs to recipients has High Throughput Screening of a 100,000 buy Dabigatran Compound Library for Inhibitors of Inenza A Virus (H3N2) William Severson 1 , ?, Michael McDowell 1 , Lynn been raised. Therefore, we have evaluated various reverse tran- scriptase (RT) inhibitors for their inhibitory effect on PERV replication in vitro. Materials and methods: The PERV-producing cell line Rasumussen 2 , Mindy Sosa 2 , Subramaniam Ananthan 3 , PK15 and the primary kidney cells from the Kagoshima strain James Noah 1 , Lucile White 2 , Colleen Jonsson 1 were used for the sources of PERV.
The human cell line 293T 1 Department of Biochemistry and Molecular Biology, AL, USA; 2 High Throughput Screening Center, AL, USA; 3 Department of Chemistry, Southern Research Institute, Birm- ingham, AL 35205, USA was infected with PERV and cultured in the presence of various concentrations of test compounds. After a certain incubation period, the amount of proviral DNA in the infected cells was determined by real-time PCR. The triphosphate (TP) forms of some compounds were also examined for their inhibitory effect There are a limited number of antivirals for the control of on PERV RT activity. seasonal and pandemic inenza viruses. To discover small Results: Among the salt pork eight RT inhibitors examined, AZT molecules that inhibit the cytopathic effect exerted by inenza was found to be the most active against PERV. Its viruses, we employed our high-throughput cell-based assay (Noah et al., Antiviral Res. 2006) to screen a commercially EC 50 was approximately 0.02 M. The order of potency was AZT > PMEO-DAPy = PMPDAP > PMPA 鈮?PMEA > d4T available compound library. Using the r