8 ± 0.7 3.8 ± 0.6 3.7 ± 0.7 Selleckchem Staurosporine * 3.8 ± 0.5 *   PL 3.3 ± 0.7 3.5 ± 0.7 3.0 ± 1.0 3.3 ± 0.7 SUP = Supplement; PL = Placebo; * = significant difference between SUP and PL. All data are reported as Mean ± SD. The anaerobic power measures are depicted in Table 2. No between group differences in any performance measure were seen at any testing period and no differences in the average performance measure were seen between SUP and PL. Significant differences in reaction time were seen between the groups. The average number of successful hits to target was JAK inhibitor review significantly higher for SUP than PL (see Figure 3a), and the average percentage of successful hits on target was also significantly greater for SUP than PL (see

Figure 3b) Figure 3 a: Reaction time: Average number of hits. * = Significant difference (p < 0.05) between the supplement and placebo. b: Reaction time: Average percentage of successful hits from total possible targets. * = Significant Trichostatin A in vivo difference

(p < 0.05) between the supplement and placebo. Data are reported mean ± SD. Table 2 Anaerobic power measures Variable Group T1 T2 T3 AVG Mean Power (W) Sup 665 ± 19 675 ± 27 686 ± 35 675 ± 23   PL 671 ± 32 684 ± 36 684 ± 43 680 ± 33 Mean Power (W/kg) Sup 7.7 ± 1.1 7.8 ± 1.2 7.9 ± 1.2 7.8 ± 1.2   PL 7.7 ± 1.1 7.9 ± 1.2 7.8 ± 1.1 7.8 ± 1.1 Peak Power (W) Sup 1099 ± 107 1097 ± 107 1098 ± 113 1098 ± 101   PL 1094 ± 76 1085 ± 111 1075 ± 120 1084 ± 95 Peak Power (W/kg) Sup 12.4 ± 1.4 12.0 ± 1.2 12.1 ± 1.7 12.2 ± 1.3   PL Mirabegron 12.5 ± 1.4 12.3 ± 1.2 12.2 ± 1.5 12.3 ± 1.3 Time to Peak Power (Sec) Sup 4.0 ± 0.1 4.0 ± 0.1 4.3 ± 1.0 4.1 ± 0.3   PL 4.1 ± 0.4 4.2 ± 0.6 4.2 ± 0.4 4.2 ± 0.3 Rate of Fatigue (W/sec) Sup 31.1 ± 5.5 30.5 ± 7.4 29.5 ± 8.4 30.4 ± 6.4   PL 31.0 ± 4.9 30.9 ± 5.8 31.2 ± 6.1

31.1 ± 5.0 Total Work (J) Sup 13300 ± 401 13500 ± 546 13713 ± 694 13515 ± 468   PL 13432 ± 599 13678 ± 719 13683 ± 861 13598 ± 651 SUP = Supplement; PL = Placebo; All data are reported as Mean ± SD. Discussion The results of this study indicate that a pre-exercise energy drink containing anhydrous caffeine, beta-alanine, vitamin C, evodiamine, N-acetyl-L-tyrosine, hordenine, 5-hydroxytryptophan, potassium citrate, N-methyl tyramine, sulbutiamine, vinpocetine, yohimbine HCL, and St. John’s wort extract can significantly improve reaction time and enhance self-perceived feelings of energy and focus. In addition, a trend towards improved alertness in subjects using this supplement versus placebo was also seen. Supplement ingestion did not have any ergogenic benefit for anaerobic power performance. Caffeine is a mild central nervous system stimulant, whose effects are similar to those associated with amphetamines, only much weaker [8]. It has been used as an ergogenic aid for many years, but consistent benefits have only been seen during exhaustive endurance exercise in which time to exhaustion is often improved [5, 9–11].