The proteins identified were classified according to their biological functions. Because it was impossible to determine the spot intensities

for overlapping spots, we only VX-809 datasheet quantified 161 single-protein spots. Figure 2 Representative 2D gel of soluble proteins of X. dendrorhous. Protein profile in stationary growth phase. https://www.selleckchem.com/products/XL184.html The image was obtained with PDQuest software ver. 7.1.1. The ID numbers were manually added and correspond to all non-redundant proteins identified by MALDI-TOF MS. Evaluation of multiple spots and differentially migrating proteins Proteins expressed from a single gene can migrate to multiple spots on 2D gels due to either post-translational modifications (such as chemical modification, proteolytic processing, and covalent attachment of small adducts) or artifactual modifications. It has been reported that several yeast proteins are resolved in multiple spots on 2D gels [24, 25]. JQEZ5 supplier Consistent with these findings, we identified 22 proteins that were represented by multiple spots (see additional file 2, Table S1), and approximately 10 proteins were present in more than three spots (Figure 2 and additional file 1, Fig. S1), including the stress-related proteins HSP70 (protein N°99) and ATP synthase β (protein N°82), which were previously reported to have multiple spots [26, 27], and PP1-1 (protein

N°19), a protein that regulates the cellular response in glucose starvation and stress [28]. In most cases, multi-spot proteins showed charge variations (horizontal spot patterns, Figure 2 and additional file 1, Fig. S1), which are usually due to protein phosphorylation or other post translational modifications that alter the isoelectric point of a protein [29]. Interestingly, Dichloromethane dehalogenase we found the protein, methionyl-tRNA formyltransferase (protein N°69), that had a diagonal spot pattern, which

is less frequently reported (Figure 2 and additional file 1, Fig. S1). This migration pattern agrees with the results of previous studies [24–27, 30], in which several metabolic proteins displayed distinct migration patterns. These multiple electrophoretic species could be generated by proteolytic events or could represent isoenzymes [29]; these possibilities were not further investigated in this work. Approximately 25% of the proteins identified in this study had potential posttranslational modifications or belonged to multigenic protein families. Accordingly, we studied the intensity profiles of proteins with multiple spots (Figure 3), of 22 multi-spot proteins identified 8 subgroups of proteins share similar profiles. For instance, a higher abundance of methionyl-tRNA formyltransferase and myosin-associated protein were observed at the end of the exponential phase. (Figure 3A).