Ishikawa M, Nakatani H, Hori K: AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high

adhesiveness to various abiotic surfaces. PLoS One 2012, 7:e48830.PubMedCrossRef 29. Pósfai G, Koob M, Hradecná Z, Hasan N, Filutowicz M, Szybalski W: In vivo see more excision and amplification of large segments of the Escherichia coli genome. Nucleic Acids Res 1994, 22:2392–2398.PubMedCrossRef 30. Murphy KC: Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli . J Bacteriol 1998, 180:2063–2071.PubMed 31. Martínez-Gil M, Yousef-Coronado F, Espinosa-Urgel M: LapF, the second largest find more Pseudomonas putida protein, contributes to plant root colonization and determines biofilm architecture. Mol Microbiol 2010, 77:549–561.PubMedCrossRef 32. Quandt J, Hynes MF: Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 1993, 127:15–21.PubMedCrossRef 33. Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A: Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum . Gene 1994, 145:69–73.PubMedCrossRef

34. Martinez-Morales F, Borges AC, Martinez A, Shanmugam KT, Ingram LO: Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction. J Bacteriol 1999, 181:7143–7148.PubMed Competing interests The authors have declared that no competing interests exist. Authors’ contributions MI performed most of experiments buy KPT-8602 and wrote the manuscript. KH designed the study and wrote the manuscript. Both authors have read and approved the final manuscript.”
“Background

Extended-spectrum β-lactamase (ESBL)-producing bacteria represent a major worldwide threat among drug-resistant bacteria in both hospital and community settings [1]. ESBLs are among the Ambler classes A, confer resistance to β-lactam antibiotics except cephamycins and carbapenems, and are inhibited by clavulanic acid [1]. ESBLs are often located on large plasmids that also harbor resistant genes to other antimicrobial classes with resulting multidrug-resistant isolates [2]. The first ESBLs have evolved by genetic mutation Reverse transcriptase from native β-lactamases TEM and SHV [3][4]. Recently, a novel type of ESBLs, the CTX-M enzymes, emerged worldwide, mostly from Enterobacteriaceae[5, 6]. CTX-M β-lactamases are not closely related to TEM or SHV ESBLs but share high amino-acid identity with chromosomal β-lactamases from Kluyvera spp. [7]. Now, bla CTX-M-15 is recognized as the most widely distributed CTX-M enzyme [8]. It is derived from CTX-M-3 by a substitution of Asp-240-Gly which increases its catalytic efficiency against ceftazidime [9]. bla CTX-M-15 are encoded on plasmids belonging to the incompatibility group IncF [10].