0045.08. Myriam Claeys, Olivier Leroux and Wim Bert are gratefully acknowledged for electron microscopy assistance. We sincerely thank Heroen Verbruggen {Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|buy Anti-cancer Compound Library|Anti-cancer Compound Library ic50|Anti-cancer Compound Library price|Anti-cancer Compound Library cost|Anti-cancer Compound Library solubility dmso|Anti-cancer Compound Library purchase|Anti-cancer Compound Library manufacturer|Anti-cancer Compound Library research buy|Anti-cancer Compound Library order|Anti-cancer Compound Library mouse|Anti-cancer Compound Library chemical structure|Anti-cancer Compound Library mw|Anti-cancer Compound Library molecular weight|Anti-cancer Compound Library datasheet|Anti-cancer Compound Library supplier|Anti-cancer Compound Library in vitro|Anti-cancer Compound Library cell line|Anti-cancer Compound Library concentration|Anti-cancer Compound Library nmr|Anti-cancer Compound Library in vivo|Anti-cancer Compound Library clinical trial|Anti-cancer Compound Library cell assay|Anti-cancer Compound Library screening|Anti-cancer Compound Library high throughput|buy Anticancer Compound Library|Anticancer Compound Library ic50|Anticancer Compound Library price|Anticancer Compound Library cost|Anticancer Compound Library solubility dmso|Anticancer Compound Library purchase|Anticancer Compound Library manufacturer|Anticancer Compound Library research buy|Anticancer Compound Library order|Anticancer Compound Library chemical structure|Anticancer Compound Library datasheet|Anticancer Compound Library supplier|Anticancer Compound Library in vitro|Anticancer Compound Library cell line|Anticancer Compound Library concentration|Anticancer Compound Library clinical trial|Anticancer Compound Library cell assay|Anticancer Compound Library screening|Anticancer Compound Library high throughput|Anti-cancer Compound high throughput screening| and Lennert Tyberghein for collecting the specimens. Electronic supplementary material Additional file 1: Transmission electron micrograph of vegetative Bryopsis thallus in longisection. Figure A: the outer cytoplasmic layer (ol) adjacent to the Bryopsis cell wall (cw) contains most of the organelles excluding only the chloroplasts (chl), which are present in the inner layer next to the central vacuole (cv). Magnification: × 8000, Scale bar: 3 μm. Figure B (detail of Figure A): besides

mitochondria (m), endoplasmic reticulum and vacuolar evaginations (v), endogenous bacteria (ba) are present in the outer cytoplasmic layer. Magnification: × 25000, Scale bar: 1 μm. (PDF 827 KB) Additional file 2: The marker used as a normalization and identification tool in all DGGE

analyses. This marker covers the full range of endophytic (including chloroplast) sequences previously obtained from Bryopsis samples MX19, MX90, MX164, MX263 and MX344 [3]. For each marker band, the band name (M1m, M1b, M2-M10), taxonomic identification, clone reference and accession number are represented. (PDF 913 KB) References 1. Burr FA, West JA: Light and electron microscope observations on the vegetative and reproductive structures of Bryopsis hypnoides . Phycologia 1970,9(1):17–37.CrossRef 2. Burr FA, Evert RF: Cytochemical study of wound-healing protein in Bryopsis hypnoides . Cytobios 1972,6(24):199–215. 3. Hollants J, Leroux O, Leliaert Torin 2 manufacturer F, Decleyre H, De Clerck O, Willems A: Who is in there? Exploration Rebamipide of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta). PLoS ONE 2011,6(10):e26458.PubMedCrossRef 4. Lachnit T,

Meske D, Wahl M, Harder T, Schmitz R: Epibacterial community patterns on marine macroalgae are host-specific but temporally variable. Environ Microbiol 2011,13(3):655–665.PubMedCrossRef 5. Burke C, Thomas T, Lewis M, Steinberg P, Kjelleberg S: Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis . ISME J 2011,5(4):590–600.PubMedCrossRef 6. Meusnier I, Olsen JL, Stam WT, Destombe C, Valero M: Phylogenetic analyses of Caulerpa taxifolia (Chlorophyta) and of its associated bacterial microflora provide clues to the origin of the Mediterranean introduction. Mol Ecol 2001,10(4):931–946.PubMedCrossRef 7. Goecke F, Labes A, Wiese J, Imhoff JF: Chemical interactions between marine macroalgae and bacteria. Mar Ecol-Prog Ser 2010, 409:267–299.CrossRef 8. Johnson CR, Muir DG, Reysenbach AL: Characteristic bacteria associated with surfaces of coralline algae – a hypothesis for bacterial induction of marine invertebrate larvae. Mar Ecol-Prog Ser 1991,74(2–3):281–294. 9. Mine I, Menzel D, Okuda K: Morphogenesis in giant-celled algae. In International Batimastat mouse Review of Cell and Molecular Biology. Volume 266.