Furthermore, as all sequence reads were delivered in FASTA format (SRA050786), the present study also provides a substantial genomic basis for A. subrufescens and, more generally, contributes to basidiomycete resources. Our results confirmed that the main limitation to SSR marker development now lies with the effectiveness of screening tests for marker validation rather than on the isolation process (Gardner et al., 2011; Malausa et al., 2011). This was particularly relevant in fungal species for which polymorphism was lower than in other phylogroups (Dutech et al., 2007). We have demonstrated the usefulness of the present set of microsatellite loci for A. subrufescens
for subsequent genetic diversity, fingerprinting and mapping analyses. This also may provide a valuable tool to resolve the taxonomic controversy associated with this species (Wasser et al., 2002, 2005; Kerrigan, 2005, 2007; Wisitrassameewong et al., 2012). In addition, R428 datasheet the transferability to closely related species offers opportunities to study speciation process and phylogenetic relationships within the Agaricus section Arvenses.
This research is a part of a research project funded by a bilateral cooperation between Mexico (project 115790 CONACYT) and France (project ‘AgaSub’ Oligomycin A chemical structure ANR-09-BLAN-0391). We would like to thank Sylvie Richard-Cervera for her technical assistance in microsatellite genotyping. We gratefully thank D. Royse, L.A. Parra, M. Verfaillie, D.C. Zied, R.L. Zhao, and all the other mycologists for providing useful fungal material. Special thanks go to R.W. Kerrigan who provided strains and also helpful suggestions to improve this manuscript. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“In Saccharomyces cerevisiae, genes involved in thiamin pyrophosphate (TPP) synthesis (THI genes) and the pyruvate decarboxylase structural gene PDC5 are transcriptionally induced in response
to thiamin starvation. Three positive regulatory factors (Thi2p, Thi3p, and Pdc2p) are involved in the expression of THI genes, whereas only Pdc2p is required for the expression of PDC5. Thi2p and Pdc2p serve as transcriptional activators Montelukast Sodium and each factor can interact with Thi3p. The target consensus DNA sequence of Thi2p has been deduced. When TPP is not bound to Thi3p, the interactions between the regulatory factors are increased and THI gene expression is upregulated. In this study, we demonstrated that Pdc2p interacts with the upstream region of THI genes and PDC5. The association of Pdc2p or Thi2p with THI gene promoters was enhanced by thiamin starvation, suggesting that Pdc2p and Thi2p assist each other in their recruitment to the THI promoters via interaction with Thi3p.